Regulatory

Part:BBa_K4743011

Designed by: Jen Hsien, Liu   Group: iGEM23_PTSH-Taiwan   (2023-10-08)


Yeast ADH1 +sqr composite promoter

We understand that the sqr promoter BBa_K4743019,originally from prokaryotes and sensitive to hydrogen sulfide, cannot function in yeast due to their eukaryotic nature. To address this, we fused the ADH1 promoter, which drives RNA polymerase activity in yeast, with the sqr promoter. In this arrangement, ADH1 acts as the driver, while sqr serves a regulatory function by aiding in the binding of the sqrR repressor. Nevertheless, the functionality of this composite promoter requires verification.

We proposed our hypothesis below

Our Hypothesis


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 692
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 70


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